Bioactive chemical compounds G1 and G2 were isolated, purified and identified from fruit bodies of the wood-rot polyporefungus Ganoderma applanatum collected from Tamarix aphylla trees in southern Iraq. The identification of these twocompounds by using GC-Mass and H1NMR was confirmed. Solubility of both compounds in different solvents was testedand the toxicity of these two compounds against human blood showed a negative result. The molecular weights of thepurified G1 and G2 compounds are 336 kd and 360 kd, respectively. The chemical formula of G1 is C20H34O4 which belongsto Tanin group while G2 is C21H28O2 and belongs to terpenoides group. The antimicrobial bioactivities of the purifiedcompounds against bacterial strains E. coli and S. aureus and against selected dermatophytic fungal isolates were tested usinga disc diffusion agar method. The minimal inhibitory concentration (MIC) was also applied. Purified G1 and G2 compoundsexhibited good bioactivities against the tested bacteria but did not show any activity against the selected dermatophytes.